金线莲多糖对Hep1-6细胞活性的影响

Research on the Cytoactive of Hep1-6 Cells Affected by Anoectochilus roxburghii Polysaccharides

通过采用MTS法、流式细胞术法以及构建RAW264.7/Hep1-6细胞共培养模型检测经不同浓度(0、125、250、500、1 000、2 000μg/mL)金线莲多糖(Anoectochilus roxburghii Polysaccharides, APS)处理的RAW264.7细胞和Hep1-6细胞的增殖及周期情况,研究APS对Hep1-6细胞生长的影响.直接使用APS处理Hep1-6细胞,发现不同浓度APS对Hep1-6细胞增殖无抑制作用,且对其周期无影响.由共培养模型可知,不同浓度APS先作用于RAW264.7细胞24 h后,将RAW264.7细胞及其培养液与Hep1-6细胞共培养24 h后,0、125、500μg/mL试验组与不加药无RAW264.7空白对照组相比,Hep1-6细胞受到一定抑制作用,细胞毒性为1级. 125、500μg/mL试验组与不加药含RAW264.7对照组相比,Hep1-6细胞具有显著抑制(P <0.01),但2 000μg/mL试验组中Hep1-6细胞生长率高于对照组. APS直接作用于Hep1-6对其活性无影响,而RAW264.7与Hepl-6共培养后,RAW264.7可以抑制Hepl-6的生长,加APS处理RAW264.7后再与Hepl-6共培养,一定浓度范围内可以进一步抑制Hepl-6的生长,但过高浓度的APS反而对共培养的Hepl-6生长有一定促进作用.

The MTS assay was used to detect the cell proliferation and the flow cytometry was used to detect the cell cycle that could reflect the growth of Hep1-6 cell and RAW264.7 cell which were treated by APS of different concentrations.The effect concentration was 0,125,250,500,1 000 and 2 000μg/mL.The cell co-culture model was constructed to detect the effect of RAW264.7 on the growth of Hep1-6.The date suggested that APS didnt inhibit the cell proliferation and had no effect on the cell cycle of Hep1-6 cell and RAW264.7 cell.It was found that the growth of Hep1-6 cell was inhibited when RAW264.7 cell treated by different concentrations of APS which were 0,125 and 500μg/mL for 24 hours co-cultured with Hep1-6 cell compared with the blank control group.The cytotoxicity was level 1.Hep1-6 cell were significantly inhibited in the 125,500μg/mL test group compared with the non-treated RAW264.7 cell control group(P<0.01),but the growth rate of Hep1-6 cell in the2 000μg/mL test group was higher than that in the control group.APS has no effect on Hep1-6 cell activity when APS directly treats cell.RAW264.7 cell can inhibit the growth of Hepl-6 cell while RAW264.7 cell and Hepl-6 cell co-culture.The growth of Hepl-6 cell can be further inhibited within a certain concentration range when RAW264.7 cell treated by APS firstly and then co-culture with Hepl-6 cell,but the excessive concentration of APS can promote the growth of Hepl-6 cell.