尖吻蝮蛇毒引起类DIC病因的研究

STUDY ON THE CAUSES OF DIC-LIKE SYNDROM INDUCED BY AAV

本文借助 DEAE-sephadex A-50和 Sephadex G-75柱层析从尖吻蝮蛇毒(AAV)内分离并纯化出一种类凝血酶(TLE).这种 TLE 以1.5U/Kg 体重注入麻醉狗的静脉后,观察到血凝筛选试验 Fgn、BPC、PT、TT 和血清 FDP 全部异常;但凝血因子Ⅱ(Quick 二期法)、AT-Ⅲ以及纤维结合蚤白(Fn)含量仍在正常范围内.这提示实验狗血循环内未有凝血酶形成的证据,从而说明 AAV-TLE是 AAV 中毒患者并发类 DIC 综合征的主要病因.

A thrombin-like enzyme (TLE) was isolated and purified from the venom of Agkistrodon acutus by ion exchange chromatography on DEAE-Sephadex A-50 and gel filtration on Sephadex G-75.The TLE(1.5U/kg) was given intravenously to anesthetized dogs .Although the coagulation screening tests (Fgn,BPC,PT,TT and serum FDP) were all abnor- mal,the two-stage plasma prothrombin,antithrombin-Ⅲ and fibronectin levels remained at normal range.These data suggest that there are no evidences of thrombin formation in the systemic circulating blood in the dogs.Thus,it is considered that the AAV-TLE is the major cause of Die-like syndrome induced by AAV.