DNA倍体分析在良恶性胸腹腔积液鉴别诊断中的应用价值

Application value of DNA ploidy analysis in differential diagnosis of benign and malignant pleural/peritoneal effusion

目的探讨DNA倍体分析在良恶性胸腹腔积液的鉴别诊断中的应用价值。方法纳入胸腹腔积液标本150例,均经影像学、实验室、病理组织学检查以及临床表现明确诊断,将恶性胸腹腔积液70例纳入恶性组,将良性腹腔积液80例纳入良性组,每例标本均同时进行脱落细胞学检查和DNA倍体分析,计算两种方法的灵敏度、特异度、阳性预测值、阴性预测值和准确度。结果 DNA倍体分析的灵敏度显著高于脱落细胞学检查,差异有统计学意义(P <0.05),阴性预测值和准确度高于脱落细胞学检查,特异度和阳性预测值低于脱落细胞学检查,但差异均无统计学意义(P>0.05)。结论在良恶性胸腹腔积液的鉴别诊断中,DNA倍体分析的灵敏度显著高于脱落细胞学检查,且保证了较高的特异度和准确度,临床应用价值更高。

【Objective】To explore the application value of DNA ploidy analysis in differential diagnosis of benign and malignant pleural/peritoneal effusion.【Methods】A total of 150 specimens were enrolled.All the patients received definitive diagnosis according to imageology,laboratory and histopathology examination combined with clinical manifestation.Seventy cases with malignant pleural/peritoneal effusion were enrolled in malignant group,while 80 cases with benign pleural/peritoneal effusion were enrolled in benign group.Each specimen received exfoliocytology examination and DNA ploidy analysis.The sensitivity,specificity,positive predictive value,negative predictive value and accuracy between two groups were calculated.【Results】The sensitivity of DNA ploidy analysis was obviously higher than that of exfoliocytology examination(P<0.05).The negative predictive value and accuracy of DNA ploidy analysis were higher than those of exfoliocytology examination,while the specificity and positive predictive value of DNA ploidy analysis were lower than those of exfoliocytology examination,but there was no statistic difference(P>0.05).【Conclusion】In differential diagnosis of benign and malignant pleural/peritoneal effusion,compared with exfoliocytology examination,DNA ploidy analysis had higher sensitivity and satisfactory specificity and accuracy.It may have higher application value.