摘要
Seven McAbs raised against human sperm were evaluated by several immunological assays. The results are shown as follows: (1) Among 7 MeAbs, 5 were IgG1, 2 were IgM.(2) By immunofluorescent assay, McAb 21-2-4 was localized on postacrosome, neck and midpiece of human sperm; other 5 McAbs were bound to plasma membrane and acrosome; another McAb gave a negative reaction. In addition, when McAbs were reacted with sperms of other species, positive results were also obtained, but with different patterns of staining. It is suggested that the location of the same antigen on sperms of various species may be different. (3) Five McAbs showed mixed pattern of agglutination in TAT, other two displayed tail-tail pattern; three McAbs gave pasitive test in SIT, two revealed weak positive reaction. (4) Cross-reactivity of seven McAbs was examined with seven human somatic tissues as well as peripheral blood lymphocytes, three McAbs reacted with lymphocytes of spleen, thymus and peripheral blood. (5) The reaction of McAbs with testes of various species was observed. McAb 21-2-4 reacted with germ cells of rabbit and hamster, and A 14 with germ cells of rat and hamster. Negative reaction of these two MeAbs with human testes was obtained, but they did react with ejaculatory, sperms, it is suggested that antigens reacted with McAbs probably, were coating antigens.
