摘要
The crossreactivities of McAbs against human sperm with trophoblast antigens were determined with Bio-dot assay,IIF and Western blotting assay.The results showed that 6 out of 8 McAbs to human sperm including A14,26-4,C43,B33-1,B33-2 and C13-2 could crossreact with trophoblast antigens obtained from microvilli of early gestation in both Bio-dot assay and IIF.The results of Western blotting assay showed that 4 out of 8 McAbs tested,i.e.C43,B33-1,B33-2 as well as C13-2 could bind to trophoblast antigens.These McAbs exhibited hinding to more than one protein band of trophoblast antigen.Based on above findings,it is clear that there are common antigens existing both in sperm cells and early trophoblast tissues.It is speculated that the tissues of early embryo also expressed in some degree determinants of germ antigens.
