摘要
对小蘖胺（Ｂｅｒｂａｍｉｎｅ）的抗氧化作用和免疫抑制作用进行了研究，发现小蘖胺（２５ｍｇ／ｋｇ／ｄ）腹腔给药一周能明显降低小鼠肝脏过氧化脂质的含量，提高血及心肌内超氧化物歧化酶的活性，并能明显减低同种异型抗原诱导的迟发型超敏反应和混合淋巴细胞反应。皮肤移植试验表明小蘖胺５０ｍｇ／ｋｇ／ｄ可以延长小鼠对异基因皮肤移植的排斥时间。用流式细胞仪对小鼠脾脏Ｔ淋巴细胞亚群分析结果表明：小蘖胺能降低小鼠脾脏ＣＤ＋４／ＣＤ８＋的比例。说明小蘖胺对小鼠免疫功能的抑制作用可能在于改变Ｔｈ／Ｔｓ的细胞平衡，使抑制活性增强，从而发挥其对免疫反应的负调节作用。
n this paper, the immunosuppressive effect and antioxidative effect ofBerbamine were studied.We found that Berbamine with the dose of 25mg/kg.7days, ip, could markedly decrease the amount of hepatic lipid peroxida-tion and enhance SOD activity of peripheral blood and cardiac muscle inmice, it also could decrease the responses of delayed-type supersensitivereaction and that of mixed lymphocytes reaction induced by allogenic anti-gen .The test of allogenic skin transplantation showed that Berbamine ( 50mg/kg.7days)could prolong the surviving time of grafting in mice.Further-more,it showed that Berbamine could also decrease the ratio of CD4+/CD8+subsets of murine spleen T lymphocytes by FACS analysis which might beone of the mechanism of its immunosuppressive effect.
出处
《中国实验动物学报》
CAS
CSCD
1995年第1期32-35,共4页
Acta Laboratorium Animalis Scientia Sinica
