VEGF-C及受体mRNA的表达与口腔鳞癌淋巴管生成的关系

The Relationship Between VEGF-C, flt-4mRNA Expression and Lymphangiogenesis of Oral Squamous Cell Carcinoma

目的探讨VEGF-C及受体在口腔鳞癌淋巴管生成中的作用。方法采用RT-PCR方法,检测47例口腔鳞癌组织标本、15例正常口腔黏膜VEGF-C、flt-4的表达;应用酶组织化学方法检测癌周微淋巴管密度(MLVD)。结果口腔鳞癌VEGF-C、flt-4mRNA的表达率分别为57.4%、61.7%,显著高于正常对照组的20%、20%(P<0.025);口腔鳞癌MLVD为14.04±6.92,显著高于正常对照组的5.48±2.62(P<0.001);VEGF-C、flt-4阳性组中,MLVD分别为17.38±4.90和17.34±4.69,显著高于阴性组的9.54±3.79和8.72±2.99(P<0.001),且VEGF-C、flt-4mRNA的表达具有显著相关性。结论VEGF-C、flt-4在口腔鳞癌癌周淋巴管生成中具有重要作用。

Objective To probe into the function of VEGF-C, flt-4 in lymphangiogenesis in oral squamous cell carcinoma.Methods VEGF-C, flt-4 expression was detected with RT-PCR method in 47 cases of oral squamous cell carcinoma and 15 cases of normal oral mucosa. The microlymphatic vessel density was detected with enzyme-histochemical reaction.Results The percentages of tumors with higher expression compared to the mean values of normal tissues were 57.4% for VEGF-C, 61.7% for flt-4(P<0.025). Significant positive relationship was found between VEGF-C and MLVD, flt-4 and MLVD(P<0.001). The mean MLVD was 14.04±6.92 in tumor group, 5.48±2.62 in normal group. There was significant difference in two group(P<0.001). Conclusion VEGF-C, flt-4 gene may play a key role in lymphangiogenesis of oral squamous cell carcinoma.