血管内皮细胞生长因子对A系小鼠胚腭突细胞增殖代谢的影响

The Effects of VEGF on the Proliferation and Metabolism of A/J Mouse Embryonic Palatal Cells

目的研究不同剂量血管内皮细胞生长因子(VEGF)对A系小鼠胚腭突细胞增殖和DNA、蛋白质及PGE2合成的影响。方法实验分为9组,每组设平行4孔(瓶),分别加入VEGF,使其终浓度为0.005ng/ml、0.01ng/ml、0.05ng/ml、0.1ng/ml、0.5ng/ml、1ng/ml、5ng/ml、10ng/ml、50ng/ml。每组均设空白对照。在实验后第1、3、5天分别检测细胞的OD值、DNA、蛋白质和PGE2的含量。结果随着培养时间的增长,VEGF促进DNA及蛋白质合成,明显刺激A系小鼠胚腭突细胞的分裂增殖。VEGF对腭突细胞PGE2的合成具有双重效应,即加入VEGF后,培养早期促进PGE2的合成;而在VEGF作用后期,则抑制腭突细胞PGE2的合成。结论VEGF促进腭突细胞的增殖,与其他生长因子一起,共同调节腭突的发育。

Objective To study the effects of vascular endothelial growth factor (VEGF) with different concentration on the OD, DNA, protein and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC). Methods Nine experiment groups, each with 4 pores (or bottles), were divided with 9 gradient concentration of VEGF (0.005,0.01,0.05,0.1,0.5,1,5,10,50 ng/ml), then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days, 5 days. Results VEGF stimulated the synthesis of DNA, protein and the proliferation of A/J MEPC. Its effects were the strongest for proliferation and Metabolism of A/J MEPC when the concentration was 10ng/ml. In early stage of culture, TGFβ1 stimulated PGE2 synthesis, but in late stage, this effect turned to inhibite PGE2 synthesis of A/J MEPC. Conclusions VEGF can stimulate proliferation and metabolism of palatal presses, and may be modified by other local growth factors.