重组人甲状旁腺激素对SLA纯钛表面MC3T3-E1细胞黏附的影响

Effects of rhPTH(1-34) on adhesion of MC3T3-E1 osteoblasts cultured on titanium surfaces of SLA

目的体外研究重组人甲状旁腺激素[rh PTH(1-34)]对MC3T3-E1细胞在大颗粒喷砂酸蚀(SLA)处理纯钛表面黏附的影响。方法通过CCK8法确定rh PTH(1-34)的最佳作用浓度,将MC3T3-E1细胞分为用含最适浓度rh PTH(1-34)培养基处理的实验组和用空白培养基处理的对照组,并分别接种于SLA处理的钛片上。用DAPI免疫荧光染色法、扫描电镜、RT-PCR对钛片表面黏附的MC3T3-E1细胞进行细胞计数,观察其形态学变化并测定MC3T3-E1细胞整合素α1、α5、β1 mRNA的表达情况。结果实验组成骨细胞的黏附数量较对照组多,肌动蛋白微丝的重构、细胞形态变化以及在钛片上黏附与伸展的速度较对照组更快,骨细胞整合素亚基α1、α5、β1表达高于对照组。结论 rh PTH(1-34)能促进MC3T3-E1细胞在纯钛表面的的黏附。

Objective To evaluate the effects of the human recombinant parathyroid hormone [rhPTH(1-34)]on the initial adhesion of MC3 T3-E1 osteoblasts cultured on titanium surfaces of sand-blasted large grift acid-etched(SLA). Methods The optimal concentration of the rh PTH(1-34) action was determined by the CCK8 method.Then the MC3 T3-E1 cells were divided into experimental groups treated with the optimal concentration of the rhPTH(1-34) medium and control groups treated with blank medium,and inoculated on SLA-treated titanium plates.DAPI immunocytochemistry,scanning electron microscopy,and RT-PCR were used to count the cell adhesion of MC3 T3-E1 cells on the surface of titanium plates. Morphological changes were observed and the expression of the integrin α1,α5,β1 mRNA in MC3 T3-E1 cells was determined. Results Compared with the control group,the adherence of osteoblasts was more than that of the control group. The remodeling of actin filaments,the change of cell morphology,and the speed of adhesion and extension on the titanium plate were faster than that of the control group. The expression of the osteoblast integrin subunit α1,α5 and β1 was higher than that of the control group.Conclusion rhPTH(1-34) can promote the adhesion of MC3 T3-E1 cells on pure titanium surface.