晚期血吸虫病患者肝脾Ⅰ、Ⅲ型胶原含量的变化

Content Change of CollagenⅠ, Ⅲ in Liver and Spleen of Patients with Advanced Schistosomiasis Japonica

目的 研究晚期血吸虫病 (晚血 )患者肝、脾组织Ⅰ型胶原 (C Ⅰ )和Ⅲ型胶原 (C Ⅲ )的含量变化。 方法 对 5 5例晚血患者肝活检标本和脾切除标本进行常规病理检查 ,肝、脾纤维化程度分期 ,经天狼猩红染色后 ,于偏光显微镜观察C Ⅰ、C Ⅲ分布情况。用图像分析仪计算C Ⅰ、C Ⅲ含量。 5例正常肝、脾标本作对照。 结果 患者肝组织C Ⅰ、C Ⅲ含量明显增加 (P <0 .0 1) ,肝窦中也明显增加 (P <0 .0 1) ,C Ⅰ /C Ⅲ比值明显降低 (P <0 .0 1)。随着肝纤维化程度的升高 ,肝窦C Ⅰ、C Ⅲ含量呈下降趋势 ,C Ⅰ /C Ⅲ比值则呈上升趋势。脾索C Ⅰ、C Ⅲ含量明显增加 ,C Ⅰ /C Ⅲ比值降低 (P值均 <0 .0 1)。随着脾纤维化程度的升高 ,C Ⅰ、C Ⅲ含量呈上升趋势 ,C Ⅰ /C Ⅲ比值则呈下降趋势。 结论 晚血患者肝、脾组织C Ⅰ、C Ⅲ含量增加 ,以C

Objective To study the change of the content of collagen typeⅠand collagen type Ⅲ (C-Ⅰ, C-Ⅲ) in liver and spleen of patients with advanced schistosomiasis japonica. Methods Sirius red staining was used to examine pathologically the liver and spleen biopsy materials from 55 patients at the advanced stage of schistosomiasis and 5 healthy persons as control. The stained slides were observed under polarizing microscope and results were interpreted by computer imaging analysis system. (Results) The content of C-Ⅰand C-Ⅲ in liver tissue and hepatic sinusoids increased significantly in the patients than that of the control (P<0.01), the ratio of C-Ⅰ/C-Ⅲ decreased considerably (P<0.01). With the progress of fibrosis in the liver, the content of C-Ⅰand C-Ⅲ in hepatic sinusoids decreased, and the ratio of C-Ⅰ/C-Ⅲ increased. The content of C-Ⅰand C-Ⅲ in spleen tissue also increased significantly in schistosomiasis patients than that of the control (P<0.01), the ratio of C-Ⅰ/C-Ⅲ decreased (P<0.01). The C-Ⅰand C-Ⅲ content increased with the progress of spleen fibrosis while the ratio of C-Ⅰ/C-Ⅲ decreased. Conclusion The result shows that the content of C-Ⅰand C-Ⅲ increased in liver and spleen of the patients with advanced schistosomiasis japonica, the increase of C-Ⅲ was more significant than that of C-Ⅰ.