珍珠岩液体培养基制备镰刀菌素C的研究

METHOD OF PRODUCING FUSARIN C IN PERLITELIQUID CULTURE MEDIUM

串珠镰刀菌可利用羟基脯氨酸、蔗糖、甘油和珍珠岩(P)等组成的 P 液体培养基合成镰刀菌素 C(Fc),其最高量为936mg/kg 有机物,比在玉米渣培养基中形成的 Fc 量较高。用 P液体培养基制备 Fc,受蔗糖浓度、胺类和培养温度及培养时间等的影响。实验证明,由 (?)g 羟基脯氨酸、40g 蔗糖和珍珠岩组成的 P 液体培养基,在28℃培养二周是形成 Fc 的理想条件。液体培养基中加入珍珠岩,Fc 的形成量增加500多倍。

For researching the biosynthesis-labelledFusarin C(Fc)by Fusarium moniliforme,amore quick and convenient method of Fu-sarin C production and purification were es-tablished,and a good liquid culture mediumconsisted of different kinds organic matters(hydroxy proline,sucrose and glycerin),in-organic salts and perlite replaced corn gritmedium.The perlite-liquid culture mediuminoculated with the strain of F.moniliformeyields 936mg Fc/kg organic matter with in 14days of incubation at 28℃.As compared withthe corn grit medium,the amount of Fc fromperlite-liquid mtdium was more than thatfrom corn grit medium(831mg Fc/kg corngrit).In all experiments both thin-layer ch-romatography and high-pressure liquid chro-matography were used to confirm the presen-ce of Fc.parameters which were importantfor the optimal biosynthesis of Fc includedhydroxy proline and sucrose concentrations,incubated time/temperature and amount ofperlite.The 40g of sucrose/L liquid culturewas optimal concentration for Fusarin C pro-duction.Of three contained N-matter tested,hydroxy proline was the best sources of N-atom for Fusarin C.Under the absence of hy-droxy proline,the Fc wasn't synthesized inperlite-liquid culture medium by F.monili-forme.A culture time/temperature study ofFc production was done,and the optimal Feamounts was synthesized after incubation for14 days at 28℃ on perlite-liquid culture me-dium.