大尺蠖核型多角体病毒多角体蛋白基因的结构分析

STRUCTURAL ANALYSIS OF POLYHEDRIN GENE IN BUZURA SUPPERISSARIA NUCLEAR POLYHEDROSIS VIRUS GENOME

大尺蠖核型多角体病毒(BsNPV)的多角体蛋白基因(ocu)定位在2.3kb 的 BamHI-H片段上,用 Xhol、Smal、HindⅢ和 PstⅠ构建了 H 片段的物理图谱,并测定了两端636bp 序列。在5′端发现了杆状病毒 ocu 基因共有的典型特征,即:ATG 起始区;启动子区(14bp保守序列);TATA box 和 CATA box 区等。单一 Xhol 位点在 ATG 上游-15bp 处,适于作为构建 ocu 基因转移载体的插入位点。在这一基因5′端序列中发现了五个反转重复单元CGAGC GCTCG,讨论了这一单元在杆状病毒 ocu 基因高效表达中的调控功能。

The polyhedrin gene(ocu)of Buzurasupperissaria nuclear polyhedrosis virus(Bs-NPV)was mapped on BamHI-H fragmentwere sequenced by Sanger ddNTP chain-ter-fragment was constructed by Xhol,Smal,Hind(?),Pstl and its 636 bp on both endswere sequenced by Sanger ddNTP chain-ter-mination method.The typical properties ofbaculovirus ocu genes are found on 5'end ofBsNPV ocu gene,which are ATG start re-gion,14 bp conservative sequences,TATAbox and CATA box etc.The unique Xholsite,which will be inserting site in transfervector constructed by BsNPV ocu gene,ismapped on-15 bp upstream of ATG.Spesific5 boxes that are CGAGC sequences are obser-ved on the gene.The important function inhigh expression of the gene is discussed.