摘要
参照已知数种固氮菌 nifA 基因的 DNA 序列,选择其中间区域的两个保守性较强的序列合成引物,利用肺炎克氏杆菌(Klebsiella pneumoniae)、棕色固氮菌(Azotobacter vinela-ndii)、巴西固氮螺菌(Azospirillum brasilense)、草螺菌(Herbaspirillum seropedicae)和深红红螺菌(Rhodospirillum rubrum)的总 DNA 进行聚合酶链反应,结果均扩增出约450bp大小的片段,经证实为各种固氮菌的 nifA 基因部分片段。核酸印迹分子杂交结果显示出nifA 基因在不同固氮菌中的同源性不强。
According to the published sequences of several nitrogen-fixing bacterial nifA gene,twohighly conservative sequences in the central domain of nifA were selected and synthesized as pri-mers.Fragments of nifA were amplified by polymerase chain reaction method,in which the totalDNA of Klebsiella pneumoniae,Azotobacter vinelandii,Azospirillum brasilense,Herbaspirillumseropedicae and Rhodospirillum rubrum were used as templates respectively.Fragments is about450bp were amplified in each species.These products were comfirmed as nifA gene fragment ofabove species.The result of Southern hybridization demonstrated that nifA gene appeared weakhomology among various nitrogen-fixing bacteria.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第5期309-313,共5页
Acta Microbiologica Sinica
基金
“863”经费资助项目
关键词
固氮菌
NIFA基因
聚合酶链反应
Nitrogen-fixing bacteria
nifA gene
Polymerase chain reaction
