醋酸钙不动杆菌产生的耐热碱性脂肪酶的研究

STUDIES ON THERMOSTABLE ALKALINE LIPASE IN ACINETOBACTER CALCOACETICUS

从福建省福州市郊区土壤中分离筛选到一株活力强的作用温度高的碱性脂肪酶野生型菌株:醋酸钙不动杆菌 F-1903。该菌产酶培养基组成（%）:大豆粉2.0、玉米浆1.0、糊精1.0、磷酸氢二钾0.5、硝酸钠0.5.产酶最适条件:发酵培养基起始 pH9.0,温度26℃,周期28小时。酶作用最适温度54℃,最适 pH9.2。Ca2+对酶有激活作用,EDTA 有抑制作用。

A bacteria strain F-1903 was isolated from Fujian province soil,which produced alka-line lipase with high activity and high activiting temperature.The medium for the strain to produce lipase was composed of(%):soy bean meal 2.0,corn-steep liquor 1.0,dextrin 1.0,K_2HPO_4 0.5,NaNO_3 0.5.The optimal conditions for lipaseproduction were initial pH 7.0,culture temperature 26℃ for 28h.The enzyme activity is optimal at pH 9.2 and at 54℃,which was increased by C(?)^(?),while inhibited by EDTA.