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提高人重组IL-6在真核细胞中表达的新途径 被引量：3

The Novel Procedures to Increase the Expression Efficiency of Recombinant Human IL-6 in Eukaryotic Cells
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摘要 为提高IL-6在真核系统中的表达效率,我们在构建真核表达载体pcDNA3IL-6的基础上,用表达载体pCI中的CMV启动子和下游所带的β-球蛋白的第一个内含子,置换了pcDNA3中的CMV启动子,构建了一种新的表达载体,使IL-6的表达水平提高了7.5倍。此外,我们还研究了pAdVAntage共转染对IL-6表达的影响,使之表达水平提高了3.6倍。以上结果为细胞因子在真核系统中的高效表达提供了有益的经验。 In order to improve the expression efficiency of recombinant IL^6 in eukaryotic cells, we constructed a new vector pcDIIL-6 to express IL-6 in eukaryotic cells on the basis of an eukaryotic vector pcDNA3IL-6 by introducin g the first intron of humanβ-globin gene into the downstream of CMV promoter in the pcDNASIL - 6. The IL-6 level expressed by pcDIIL - 6 is 7. 5 times higher than that of pcDNA3IL - 6. On the other hand , we studied the influence of pAdVAntage vector co - transfection on the expression level of IL-6 in eukaryotic cells and found the level of IL-6 is 3. 6 times higher than that of pcDNA3IL - 6. The results we got are beneficial to express cytokines with high efficiency in eukaryotic cells.
作者 刘红涛 袁勇 张颖妹 王应 狄春辉 马大龙
机构地区 北京医科大学免疫系
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 1996年第2期135-139,共5页 Chinese Journal of Cancer Biotherapy
关键词 人重组IL-6 真核细胞 表达 基因转染 细胞因子 Interleukin 6 gene transfection intron COS-7 cell
分类号 R392.3 [医药卫生—免疫学]
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  • 8Martel Pelletier J, McCollum R, DiBatfista J, a al. The interleukin-1 receptor in normal and osteoarthritic human articular chondrocytes. Identification as the type I receptor and analysis of binding kinetics and biologic function[J]. Arthritis Rheum, 1992,35(5) :530 - 540.
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中国肿瘤生物治疗杂志
1996年 第2期

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