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细小病毒非结构蛋白基因转染对胃癌细胞株细胞因子表达的影响 被引量:1

The Effects of Parvoviral Nonstructural Proteins Gene Transfection on the Expression of Cytokines in Gastric Cancer Cell
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摘要 将可诱导表达的细小病毒非结构蛋白基因转染入人低分化胃腺癌细胞株MKN-45,该基因诱导表达后部分宿主细胞死亡,成活细胞生长特性发生显著改变,如细胞间粘附力增强,倍增时间延长,裸鼠体内成瘤能力下降。RNA斑点印迹显示非结构蛋白基因的表达能明显增加胃癌细胞白细胞介素-1α,白细胞介素-1β和白细胞介素6核因子表达,而对白细胞介素6表达无影响。提示细小病毒的抗肿瘤作用部分可能是由其非结构蛋白影响肿瘤细胞的细胞因子表达所介导的。 Human gastric cancer cells (MKN-45) were transfected with pULB3238 (a plasmid carrying MVMp NS gene). After the NS gene expressed, some of the NS-transfectants were died. The other cells were survived. In comparison with those of control MKN-45, the survived NS- transfected MKN-45 cells had the following characterizations: (a). nucleus/cytoplasm ratio reduced; (b). generation time prolonged;(c). adhesion ability between cells increased;(d). cloning efficiency decreased;(e). formation of tumors in nude mice suppressed. To investigate the possible mechanisms of above mentioned change of NS-transfectants, the effect of NS on the expression of some cytokine genes were explored. RNA dot blot indicated that the NS might enhance the expression of IL-lα(2.7 times), IL-1/β(4.6 times) IL- 6 nuclear factor (2.5 times) and had no effect on the expression of IL-6. The results suggested that the antineoplastic activity of parvoviruses may be medicated by NS interfere with the expression of cytokines in the host cells.
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 1996年第3期203-206,共4页 Chinese Journal of Cancer Biotherapy
关键词 细小病毒非结构蛋白 基因转染 胃癌 细胞株 细胞因子 表达 癌细胞 parvoviral nonstructural proteins gene transfection cytokine.
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同被引文献32

  • 1林万敏,徐磊,马承武,万山红,罗祖玉.自发转化的人胎肝细胞株对细小病毒H-1感染敏感性的变化[J].细胞生物学杂志,1994,16(2):69-72. 被引量:2
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