苯乙酸钠增强肿瘤细胞表面HLA分子的表达

Sodium Phenylacetate Enhances HLA Molecule Expression on the Surface of Tumor Cells

以分化绣导剂苯乙酸钠处理肿瘤细胞,以ELISA检测肿瘤细胞表面HLA Ⅰ、Ⅱ类分子表达的变化。结果发现MCF-7、MDA-453、MKN-45以及Hela细胞表面HLA Ⅰ类分子表达较低,而MKN-28和3AO细胞表面HLA Ⅰ类分子高表达。MDA-453、MKN-28、MKN-45、Hela以及3AO细胞表面亦表达HLA Ⅱ类分子,而MCF-7细胞表面缺乏HLA Ⅱ类分子。苯乙酸钠能够诱导MCF-7细胞表面表达HLA Ⅱ类分子;增强MCF-7细胞表面HLA Ⅰ类分子,MDA-453、MKN-28、MKN-45、Hela以及3AO细胞表面HLA Ⅰ、Ⅱ类分子的表达。并且肿瘤细胞表面HLA Ⅰ类分子的表达与苯乙酸钠的作用时间和剂量呈正相关。

Sodium phenylacetate can induce differentiation of tumor cells and has been approved as a drug for the treatment of adults with cancer. In order to explore the immunological mechanism of its antitumor effect, the influence of sodium phenylacetate on HLA class I and II molecule expression in various human tumor cell lines, including breast adenocarcinoma (MCF-7.MUA-453), gastrocarcinoma(MKN-28,MKN-45), ovarian cancer(3AO) and cervical cancer(Hela), was studied with ELISA. The result showed that HLA class II molecule was absent from the surface of MCF-7 cells, but they could be induced after 7 days of continued treatment with sodium phenylacetate. Sodium phenylacetate was found to increase HLA class I molecule expression on the surface of MCF-7 cells, HLA class 1 and II molecule expression on the surface of MDA-453、 MKN-28、 MKN-45、 Hela and 3AO cells. The effect of sodium phenylacetate on HLA class I molecule expression in tumor cells is dose-and time-dependent.