摘要
金合欢根瘤菌Rhizobium sp.(Acacia farnesiana)的氧化酶、过氧化氢酶、脲酶、青霉素酶和β-半乳糖苷酶均呈阳性。经过酶活性定量测定表明金合欢根瘤菌有葡萄糖酸-6-磷酸脱氢酶(6PGD)和β-半乳糖苷酶的酶活性,而慢生型大豆根瘤菌未测到上述两种酶活性。根据聚丙烯酰胺凝胶电泳酯酶图谱,6株金合欢根瘤菌可分成两群:酋株AF1、AF2和AF3的酯酶图谱中有A带,AF4、AF5和AF6的酯酶图谱中没有A带。
Rhizobium strains isolated from Acacia farnesiana were positive for oxidase, catalase, urease, penicillinase and β-galactosidase. The quantitative determination of enzymatic acti- vity indicated that these strains possessed 6-phosphogluconate dehydrogenase (6PGD) and β- galactosidase activities but Bradyrhizobium japonicum didn't possess 6PGD and β-galactosi- dase activities. Six strains isolated from A. farnesiana may be divided into two groups according to esterase pattern on PAGE: strains AF 1, AF2 and AF3' possessed A band on esterase pattern but strains AF4, AF5 and AF6 didn't.
出处
《微生物学通报》
CAS
CSCD
北大核心
1992年第1期9-12,共4页
Microbiology China
关键词
金合欢根瘤菌
6PGD
Β-半乳糖苷酶
Rhizobium isolated from Acacia farnesiana
6PGD
β-galactosidase
Esterase pattern
