摘要
OBJECTIVE Bisphenol A(BPA)is a commonly used phenolic environmental estrogen that impacts male reproductive system.Cistanchetubulosa(CT)is a traditional Chinese medicine used to boost male sexual activity.It has been proved for increasing the expression of steroidogenic enzyme and remarkable antioxidant activity.This study aimed to investigate the mechanisms behind the possible protective effects of CT against BPA-induced testicular and sperm toxicity in rats.METHODS CT was identified by 5.8sgene sequencing.The major compositions(echinacosideand acteoside)of CT were quantified by HPLC method.CT(200or 400mg·kg-1)or VE(positive control,300mg·kg-1)and BPA(200mg·kg-1)were administered orally(one time per day)to adult male ratsfor 6weeks.The sperm parameters were observed by dark-field microscope;serum hormone levels(FSH,LH and T)were tested by radio immunosorbent;LDH-x activity,redox balance status(content of MDA,GSH,H2O2 and OH-,and activity of SOD)were evaluated using commercial kits;the expression of the key steroidogenic enzymes were evaluated by qRT-PCR,heat map,immunofluorescence and Western blotting.RESULTS The CT treatment reversed BPA-induced abnormal in sperm characteristics,testicular structure and LDH-x activity,as well as normalized serum testosterone.CT increased both mRNA and protein expressions of key steroidogenic enzymes including StAR,CYP11A1,3β-HSD,17β-HSD and CYP17A1,suggesting that CT enhanced sex hormone synthesis.Moreover,the remarkable antioxidant effect of CT contributed to its protection against BPA-induced testicular toxicity,which was evidenced by the normalization of testicular,redox markers(MDA,H2O2,OH-and GSH contents,and SOD activity)after CT treatment.CONCLUSION CT effectively attenuated BPA-induced poor sperm quality and testicular toxicity in rats through steroidogenesis and redox pathways.
OBJECTIVE Bisphenol A(BPA)is a commonly used phenolic environmental estrogen that impacts male reproductive system.Cistanchetubulosa(CT)is a traditional Chinese medicine used to boost male sexual activity.It has been proved for increasing the expression of steroidogenic enzyme and remarkable antioxidant activity.This study aimed to investigate the mechanisms behind the possible protective effects of CT against BPA-induced testicular and sperm toxicity in rats.METHODS CT was identified by 5.8sgene sequencing.The major compositions(echinacosideand acteoside)of CT were quantified by HPLC method.CT(200or 400mg·kg-1)or VE(positive control,300mg·kg-1)and BPA(200mg·kg-1)were administered orally(one time per day)to adult male ratsfor 6weeks.The sperm parameters were observed by dark-field microscope;serum hormone levels(FSH,LH and T)were tested by radio immunosorbent;LDH-x activity,redox balance status(content of MDA,GSH,H2O2 and OH-,and activity of SOD)were evaluated using commercial kits;the expression of the key steroidogenic enzymes were evaluated by qRT-PCR,heat map,immunofluorescence and Western blotting.RESULTS The CT treatment reversed BPA-induced abnormal in sperm characteristics,testicular structure and LDH-x activity,as well as normalized serum testosterone.CT increased both mRNA and protein expressions of key steroidogenic enzymes including StAR,CYP11A1,3β-HSD,17β-HSD and CYP17A1,suggesting that CT enhanced sex hormone synthesis.Moreover,the remarkable antioxidant effect of CT contributed to its protection against BPA-induced testicular toxicity,which was evidenced by the normalization of testicular,redox markers(MDA,H2O2,OH-and GSH contents,and SOD activity)after CT treatment.CONCLUSION CT effectively attenuated BPA-induced poor sperm quality and testicular toxicity in rats through steroidogenesis and redox pathways.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2015年第S1期100-100,共1页
Chinese Journal of Pharmacology and Toxicology
基金
The project supported by the High-end Foreign Experts Recruitment Programme of State Administration of Foreign Experts Affairs(GDW20146100228)
the Ministry of Education and State Administration of Foreign Experts Affair″Overseas Teacher″Project(MS2011XBNL057)
the Key Construction Program of International Cooperation Base in S&T,Shaanxi Province(2015SD0018)
