Effect of Weiliuan of different prescriptions on gastrointestinal regulation factor in spleen-deficiency rats

OBJECTIVE To observe the regulating effect of Weiliuan on digestive enzyme and relevant gastrointestinal hormone in spleen-deficiency rats.METHODS The rats were given the decoction of Radix et Rhizoma combine with dietary disorder to induce spleen-deficiency syndrome.Spleen-deficiency rats were divided into 8groups:normal control group,spleen deficiency model group,Mengtuoshi San group(0.15g·kg-1),Weiliuan 31.5and 15.75g·kg-1 groups,Weiliuan splited prescriptionⅠ 31.5 and 15.75g·kg-1groups,Weiliuan splited prescriptionⅡ 31.5and 15.75g·kg-1 groups.At the end of experiment,the level of amylase(AMS),lactic dehydrogenase(LDH),succinate dehydrogenase(SDH),motilin(MTL),somatostatin(SS),gastrin(GAS)and organ coefficient were detected.RESULTS Weiliuan group,Weiliuan splited prescriptionⅠ group and Weiliuan splited prescriptionⅡ group can significantly reduce the spleen index,the level of SDH and LDH,it can also improve the level of the AMS(P<0.05,P<0.01)In the regulation of hormone levels,Weiliuan group,Weiliuan splited prescriptionⅠ group and Weiliuan splited prescriptionⅡ group can significantly improve the levels MTL and GAS(P<0.05),but had no obvious effect on SS.CONCLUSION Weiliuan group,Weiliuan splited prescriptionⅠ group and Weiliuan splited prescriptionⅡ group can improve the symptoms of spleen deficiency syndrome significantly.Its mechanism may be to regulate the levels of AMS,SDH,LDH,MTL and GAS.