摘要
OBJECTIVE To investigate the effect of Urena lobataleaves extract on the inhibition of nephropathy diabetic complication.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,kidney organ were collected and then superoxyde dismutase(SOD)kidney level,malondialdehyda(MDA)and tumor necrosis factor-alpha(TNF-α)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD kidney level about 30%,60% and 90% respectively compared to diabetic group(P<0.05),while the MDA kidney level was decreased by 30%,60% and 70%(P<0.05)respectively.The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw were also decrease TNF-αkidney level approximately 30%,40% and 60% compared to control group(P<0.05).In diabetic groups,SOD kidney level was decreased compared to normal group(P<0.05)while the MDA and TNF-αwere increased(P<0.05).CONCLUSION U.lobata leaves extract could inhibit nephropathy diabetic complication by increasing of SOD kidney level,decreasing of MDA kidney level,and TNF-α.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.
OBJECTIVE To investigate the effect of Urena lobataleaves extract on the inhibition of nephropathy diabetic complication.METHODS This study uses control group post test only with male Sprague dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with water extract of U.lobataleaves in concentrations of 250,500 and 1000mg·kg-1 bw for 4 weeks.After scarifying,kidney organ were collected and then superoxyde dismutase(SOD)kidney level,malondialdehyda(MDA)and tumor necrosis factor-alpha(TNF-α)were examined.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of U.lobataleaves extract 250,500 and 1000mg·kg-1 bw were able to increase SOD kidney level about 30%,60% and 90% respectively compared to diabetic group(P<0.05),while the MDA kidney level was decreased by 30%,60% and 70%(P<0.05)respectively.The supplementation of water extract from U.lobatain dose of 250,500 and 1000mg·kg-1 bw were also decrease TNF-αkidney level approximately 30%,40% and 60% compared to control group(P<0.05).In diabetic groups,SOD kidney level was decreased compared to normal group(P<0.05)while the MDA and TNF-αwere increased(P<0.05).CONCLUSION U.lobata leaves extract could inhibit nephropathy diabetic complication by increasing of SOD kidney level,decreasing of MDA kidney level,and TNF-α.This effect may be related to active compounds that act as an antioxidant and anti-inflammatory in U.lobata extract.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2015年第S1期119-119,共1页
Chinese Journal of Pharmacology and Toxicology
基金
The project supported by Ministry Education of Indonesia