卡巴胆碱对内毒素刺激大鼠腹腔巨噬细胞炎症细胞因子表达的影响

Effects of carbachol on inflammatory cytokines expression of rat abdominal macrophages stimulated by lipopolysaccharide

目的:研究拟胆碱药卡巴胆碱在体外对内毒素(LPS)刺激大鼠腹腔巨噬细胞表达炎症细胞因子的作用。方法:采集雄性Wistar大鼠腹腔巨噬细胞,经卡巴胆碱、烟碱或毒蕈碱预处理后,再给予LPS刺激,4h后取细胞培养上清液,ELISA法检测TNF-α、IL-6和IL-10水平。结果:LPS刺激后TNF-α、IL-6和IL-10表达均显著增加。卡巴胆碱与烟碱能明显抑制TNF-α、IL-6表达的增加(P<0.01),卡巴胆碱的抑制作用有明显的量效关系,毒蕈碱的抑制作用则明显低于卡巴胆碱和烟碱(P<0.01)。卡巴胆碱和烟碱对LPS刺激后的IL-10水平升高均无明显影响。结论:卡巴胆碱在体外LPS刺激腹腔巨噬细胞炎症模型中能抑制促炎细胞因子表达的增加而对抗炎细胞因子表达无明显作用,其作用与烟碱类似。

AIM:To investigate the effects of cholinergic drug carbachol on the expression of inflammatory cytokines from rat abdominal macrophages stimulated by lipopolysaccharide(LPS).METHODS:Rat peritoneal macrophages were collected and exposed to carbachol,nicotine or muscarine,and then stimulated by LPS for 4 hours.Supernatants of cultures were harvested and assayed for TNF-α,IL-6 and IL-10 by ELISA method.RESULTS:The expressions of TNF-α,IL-6 and IL-10 were significantly increased after LPS stimulation.The increase of TNF-α and IL-6 expression was inhibited after addition of carbachol and nicotine,which was dose-dependent in carbachol group.The inhibitive effect of muscarine was much less than that of carbachol and nicotine(P<0.01).Carbachol and nicotine had no significant effect on IL-10 release stimulated by LPS.CONCLUSION:The results suggest that carbachol,similarly to nicotine,inhibits the expression of pro-inflammatory cytokines but has no effect on the expression of anti-inflammatory cytokines on inflammatory model of rats abdominal macrophages.