异补骨脂素防护人晶状体上皮细胞的凋亡

Isopsoralen inhibiting the apoptosis of human lens epithelial cells

目的:探讨异补骨脂素(ISR)对氧化损伤的人晶状体上皮细胞(HLEC-B3)凋亡有无抑制作用。方法:本研究在终浓度300μmol/LH2O2培养液中复制HLEC-B3凋亡模型,并以雌二醇(E2)作为阳性对照,在透射电子显微镜下观察HLEC-B3超微结构的改变,采用FCM法检测HLEC-B3凋亡率及线粒体膜电位的变化。结果:超微结构研究显示,H2O2组绝大多数HLEC-B3发生凋亡,并呈凋亡各期改变的全过程;E2、ISR组仅少数HLEC-B3发生凋亡,并多为早期或中期的轻微改变。FCM结果显示:H2O2组HLEC凋亡率显著高于空白对照组;E2和ISR组凋亡率均低于H2O2组(P<0.01)。H2O2组线粒体跨膜电位比对照组显著下降;E2组和ISR组的线粒体跨膜电位均比H2O2组升高(P<0.01)。结论:ISR可减轻实验性氧化损伤的HLEC-B3凋亡程度,为寻求防治老年性白内障的药物提供实验依据。

AIM:To investigate if isopsoralen inhibits the apoptosis of human lens epithelial cells(HLEC)with experimental oxidative injury(HLEC-B3).METHODS:Lens epithelial cells were incubated with 300 μmol/L H202 to make the HLEC-B3 model.The estradiol(E2)was as a positive control,the HLEC apoptosis and apoptosis rate were measured by FCM,the ultrastructure changes and apoptosis bodies of HLEC were observed under transmission electron microscope.RESULTS:The ultrastructure observation indicated that the apoptosis cells occurred in most HLEC of H202 group and the changes were severe and presenting different stages.While the changes in E2 and ISR groups were slight and most of them were in early and middle stages.The apoptosis rate in H202 group was significantly higher than that in the control group,E2 and ISR groups.The ΔΨM in H2O2 group was reduced obviously compared with control group,E2 and ISR groups.CONCLUSION:ISR may decrease the apoptosis of HLEC,providing scientific evidence for pursue effective medicine to prevent and treat senile cataract.