单纯疱疹病毒分子生物学分型方法的研究

STUDY ON HERPES SIMPLEX VIRUS TYPING WITH MOLECULAR BIOLOGICAL METHODS

联合运用HSV-1和HSV-2型特异性核酸探针对28株HSV临床分离株做了鉴定分型,并与应用HSV型特异性单克隆抗体的三种免疫学方法的检测结果进行了比较。核酸探针与单克隆抗体之间的符合率为100%,但分型率不同,核酸探针为100%,单克隆抗体为64—82%。研究结果表明,本实验所建立的HSV-McAb-APAAP桥联免疫酶染技术具有简便、敏感、实用的特点。

A total of twenty eight HSV isolates from the patients were typed by using HSV typespecific monoclonal antibodies (McAb). Comparison of typing results with McAb in three Sero-lminunological methods with molecular hybridization indicated 100% concordance in the results. But the typing rates were quite different among the various methods (ELISA 64%, IFA and EIA 82%, Hybridization 100%). The results demonstrate that the molecular hybrydization with HSV type-specific probes was highly sensitive and specific, and the method of EIA with HSV type-specific McAb was accurate, cheap, rapid and practical.