异源表达玉米ZmWRKY11基因增强拟南芥对盐胁迫的耐受性

Overexpression of a Maize ZmWRKY11 Gene Enhances Salt Tolerance in Transgenic Arabidopsis

从玉米中分离得到响应高盐胁迫的WRKY基因,将其命名为ZmWRKY11。构建过量表达载体pCAMBIA1301-ZmWRKY11,采用花序侵染法转化拟南芥,研究ZmWRKY11基因的生物学功能,扩繁后对T3代转基因植株进行耐盐鉴定试验。结果表明,转基因株系的绿苗率在盐胁迫处理后显著高于野生型拟南芥,同时,野生型株系中丙二醛含量和相对电解质渗透率相较于转基因植株发生了更为显著的上升,但脯氨酸含量的上升幅度则小于转基因株系。结果表明,ZmWRKY11在拟南芥中的异源过表达增强了转基因植物对盐胁迫的耐受性。

WRKY11 encoding a WRKY transcription factor was cloned from maize.ZmWRKY11 expression was induced by salt stress.To validate the function of ZmWRKY11,we constructed an overexpression vector pCAMBIA1301-ZmWRKY11 and transformed the vector into Arabidopsis.Heterologous overexpression of ZmWRKY11 in Arabidopsis significantly enhanced plant tolerance to salt stress,as determined by physiological indicators such as cotyledons greening rate,malondialdehyde(MDA)content,relative electrolyte leakage(REL)and proline content.These findings suggested that ZmWRKY11 may function as a positive factor in plant salt stress response.