摘要
对含有不同R质粒的6株大肠杆菌J53和不含质粒的大肠杆菌J53所产生的L-天门冬酰教酶的活力进行了比较,前者的L-天门冬酰胺酶活力比后者的降低了约1/2—3/4。消除大肠杆菌J53细胞中的R质粒后,L-天门冬酰胺酶活力明显增加并与不含质粒的大肠杆菌J53的相近。结果表明,在大肠杆菌内存在的R质粒对寄主的L-天门冬酰胺酶活力具有明显的抑制作用。
L-asparaginase activity produced by six E. coli J53 strains containing different plasmids and plasmid free E. coli J53 strain was compared. The enzyme activity of the plasmid- bearing strains was about 2—4 times lower than that of the plasmid free ones. Curing the R plasmids from E. coli J53, the activity of L-asparaginase increased and was close to that of the plasmid free strain. It is proved that the remarkable inhibition of L-asparaginase activity results from the presence of the plasmid in E. coli.
出处
《微生物学通报》
CAS
CSCD
北大核心
1992年第5期277-280,共4页
Microbiology China
