摘要
本文用正交试验设计方法选出的球形芽孢杆菌发酵培养基配方,菌体数量可达到100亿/ml,芽孢形成率达到70—80%,杀蚊幼的半致死剂量在0.125ppm以下。上述的三个指标明显优于以前的报道。通过摇瓶发酵验证和50L^3小罐的放大试验,证明所有的实验结果都很稳定。使用现在的发酵配方,使每吨发酵液的成本降低了33%。
The medium for fermentation has been selected by means of orthogonal designs. The number of cell has reached 10^(11)/ml, and spore forming rate is 70—80%. LC_(50) of mosquito larvicied is lower than 0.125 ppm. Three targets are obviously better than those of previous report. The stable results are proved by studying of fermentation in shaking flask and further in 50L^3-bench fermenter. The production cost of per ton is decreased by 33%.
出处
《微生物学通报》
CAS
CSCD
北大核心
1992年第5期261-264,共4页
Microbiology China
关键词
球形芽孢杆菌
发酵条件
Orthogonal design
Bacillus spkaericus
Fermentative condition
