从辣木籽中制备4-α-L-鼠李糖基-异硫氰酸苄酯的工艺优化研究

Optimization of Preparation Technology of 4-[(α-L-Rhamnosyloxy)Benzyl] Isothiocyanate from Moringa Oleifera Seeds

【目的】探索从辣木籽中酶解提取4-α-L-鼠李糖基-异硫氰酸苄酯(4-[(α-L-rhamnosyloxy) benzyl] isothiocyanate,GMG-ITC)的工艺条件及纯化方法。【方法】通过单因素和正交试验的方法,优化了对脱油辣木籽中硫代葡萄糖苷进行酶解提取,进而以二氯甲烷为溶剂进行萃取及重结晶可获得高纯度的GMG-ITC。【结果】通过高效液相色谱法测定酶解提取液中GMG-ITC含量,筛选出制备辣木籽GMG-ITC的最佳酶解提取工艺为:时间9 h,料液比1∶60 (g∶mL),pH值为5.0,温度30℃。【结论】建立了简化且高效的辣木籽脱油、酶解提取、萃取、重结晶4步新工艺组合,制备得到产率为4.70%、纯度达98%的GMG-ITC。

[Purpose]To optimize of enzymatic hydrolysis and purification conditions of 4-[(α-Lrhamnosylbenzyl)] isothiocyanate(GMG-ITC) from Moringa oleifera seeds.[Methods]Through single factor and orthogonal experiments, we optimized the conditions for enzymatic extraction of glucosinolates from the de-oiled M. oleifera seeds. Then, the high purity GMG-ITC by extraction and recrystallization with methylene chloride were obtained.[ Result] An optimum condition for enzymatic hydrolysis was obtained as following: material to solvent ratio 1∶60(g∶mL), temperature30 ℃, reaction time 9 h, pH 5, by HPLC analyzing.[Conclusion]We described a simple and efficient four-step process including de-oiling, enzymatic hydrolysis, extraction and recrystallization to afford GMG-ITC from M. oleifera seeds, an overall yield of 4.70% was achieved with 98% purity.