表皮细胞条件培养基对真皮干细胞的影响

The influence of keratinocytes conditioned medium on skin-derived precursors

真皮干细胞(SKPs)是一种源自真皮,在毛囊再生中发挥关键作用的多能干细胞.然而,SKPs的体外扩增是一项尚未完全解决的难题.该研究结果显示,表皮细胞条件培养基能显著促进SKPs的增殖,增加SKPs特征性基因Akp2,Sox2,Bmp4,Nestin1,PDGFa,Clstn2,Trps1,Versican的表达水平,并提高SKPs毛囊诱导能力标志性蛋白-碱性磷酸酶的活性.因此,该研究提示表皮细胞分泌的蛋白对于SKPs的增殖和干性的维持有重要作用.

Skin precursor cells(SKPs) are multipotent stem cells derived from the dermis. SKPs are able to induce hair follicle regeneration. It is considered to be seed cells for the thereby of the skin injury. Their application is limited by the instability of their property during culture expansion. In this study, we employed epidermal cell conditioned medium to treat SKPs and found that the proliferation rate of SKPs was increased. In addition, the expression levels of SKPs signature genes Akp2, Sox2, Bmp4, Nestin1, PDGFa, Clstn2, Trps1 and Versican were up-regulated. In addition, the activity of alkaline phosphatase was enhanced significantly which reflect the hair follicle inducing ability of SKPs. Our study indicates that factors released by epidermal cells facilitate the stability of SKPs properties.