摘要
通过杂交瘤技术及单克隆抗体技术获得针对禽流感病毒H7亚型的特异性单克隆抗体.抗体用于标记荧光微球,并基于双抗体夹心免疫层析法用于对禽流感病毒H7亚型的检测.针对禽流感病毒H7亚型,检出限为0.125 HAU,具有较高的灵敏度,所建立的禽流感病毒H7亚型荧光检测试纸具有良好的特异性和准确性,可用于对禽流感病毒H7亚型的现场快速检测.
The specific monoclonal antibodies against avian influenza virus H7 subtype were obtained through hybridoma technology and monoclonal antibody technology.The antibodies were used to label fluorescent microspheres and detect the avian influenza virus H7 subtype based on sandwich lateral flow immunoassay.For detecting the avian influenza virus H7 subtype,the limit of detection was 0.125 HAU with a high sensitivity.The immunoassay strips for detecting avian influenza virus H7 subtype have a good specificity and accuracy,and can be used for rapid on-site detection of the avian influenza virus H7 subtype.
作者
吴峰
卢奕良
吕琦
毛茅
岑瑜
马岚
WU Feng;LU Yi-liang;LYU Qi;MAO Mao;CEN Yu;Ma Lan(Division of Life Science&Health,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China;College of life sciences and Oceanography,Shenzhen University,Shenzhen 518055,China;School of Life Sciences,Yunnan University,Kunming 650091,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第A01期103-107,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
深圳市科技研发基金(JCYJ20160323163102764,CXZZ20150814101140575)
深圳市技术创新计划(20150529151025)
深圳市战略性新兴产业发展专项资金(S2012007890013)
关键词
禽流感病毒
H7亚型
单克隆抗体
免疫检测
avian influenza virus
H7 subtype
monoclonal antibody
immunoassay
