摘要
用鸡卵清蛋白偶联EDTA螯合的重金属汞离子、镉离子作为免疫原,采用快速免疫佐剂分别免疫Balb/C小鼠,并通过腹腔注射NS-1骨髓瘤细胞,制备抗重金属汞离子、镉离子小鼠多克隆抗体腹水.通过此方法可快速大量获得高效价的能用于重金属汞离子、镉离子免疫检测试剂开发的小鼠多克隆抗体.
OVA conjugated EDTA which chelated mercury ions,cadmium ions were used as immunogen.Multiple murine polyclonal antibodies against mercury ion and cadmium ion were produced by immunization of Balb/C mice with rapid immune adjuvant and immunogen,and injecting NS-1 myeloma cells intraperitoneally.This method can rapidly and efficiently prepare highly effective polyclonal antibodies against heavy metal mercury ion and cadmium ion for the development and application of detection kit.
作者
吴峰
吕琦
毛茅
岑瑜
马岚
WU Feng;LYU Qi;MAO Mao;CEN Yu;MA Lan(Division of Life Science&Health,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China;School of Life Sciences,Yunnan University,Kunming 650091,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第A01期108-111,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
深圳市科技研发基金(CXZZ20150814101140575)
深圳市技术创新计划(20150529151025)
深圳市战略性新兴产业发展专项资金(S2012007890013)
关键词
重金属
汞
镉
多克隆抗体
heavy metal
mercury
cadmium
polyclonal antibody
