芜菁花叶病毒(广州油菜株)生化性质的研究

STUDIES OF BIOCHEMICAL PROPERTIES OF TURNIP MOSAIC VIRUS(GUANGZHON STRAIN)

我们对芜菁花叶病毒广州油菜株(TuMV—G.Z)进行了生化性质的研究。结果表明,纯化的病毒外壳蛋白经10％SDS-聚丙烯胺酰胶电泳出,现一个组份,其分子量为1.4×10~4d;氨基酸组份分析表明,TuMV—G.Z外壳蛋白亚基由约107个氨基酸残基组成,其中包括1个色氨酸和5个酩氨酸;DNS—C1末端法测其N端,结果显示为苏氨酸,且其氨基是游离的。

10％SDS-PAGE revealed the M.W.of TuMV-G.Z coat protein is 14200 dalton.On the basis of amino acid analysis and M.W.,We conclude that the coat protein consists of about 107 amino acid residues.N-teminal of the protein determined by DNS-Cl method is threonine and nonencolsed.The virus in suspension at 4℃ is unstable.