摘要
土壤中反硝化菌和固氮菌的作用强度与其产生的N_2O及C_2H_2还原成C_2H_4的量有一定关系。本文报导了为测定土壤中微量的N_2O、C_2H_2和C_2H_2数量,所建立的双柱双检测器一次进样同时测定的二维气相色谱法。此法能简单、快速、准确地反映出土壤中反硝化菌和固氮菌的作用强度。文中对仪器装置、色谱条件、样品处理、干扰物的消除、最小检测浓度、重复性、方法应用等进行了报导。
In this paper,a simultaneous determination of trace amount of N_2O and C_H_4/C_2H_2 produced by the denitrification and nitrogen fixation in soil by two dimensional gas chromatography of dual column and dual detector was presented. A simple, fast and accurate analytical method was developed. Instrumental installation, column, conditions, sample disposal, interfering component elimination, minimum detectable concentration, repeatability and applications cf the method were reported.
出处
《微生物学杂志》
CAS
CSCD
1992年第2期14-17,共4页
Journal of Microbiology
关键词
气相色谱
反硝化菌
固氮菌
土壤
Gas Chromatography
Denitrobacteria
Azotobacteria
Action Intensity
Two Dimensional Gas chromatography