运动诱发性支气管哮喘中免疫机制的初步探讨

A study on the role of T lymphocytes and eosinophil cationic protein in exercise-induced asthma

目的 探讨嗜酸性粒细胞分泌的嗜酸细胞阳离子蛋白 (ECP)、T淋巴细胞的激活和运动诱发性支气管哮喘 (EIA)的关系。方法 选择支气管哮喘 (简称哮喘 )患者 3 2例 ,其中运动性哮喘患者 (A组 ) 13例 ,非运动性哮喘患者 (B组 ) 19例、正常对照组 (C组 ) 8名进行踏车运动试验 ,分别测定其运动前、运动后 10min、1h血清中的ECP浓度、白细胞介素 5 (IL 5 )mRNA、外周血CD+ 2 5T淋巴细胞占总淋巴细胞数的百分比 (CD+ 2 5% )。结果 (1)运动前A组患者的一秒钟用力呼气容积 (FEV1)与ECP和CD+ 2 5%呈负相关 (r分别 =- 0 79和 - 0 61,P均 <0 0 1) ;(2 )运动后A组患者FEV1为 (2 5 2±14 4) % ,PEF为 (41 2± 3 3 0 ) % ;B组患者运动后FEV1为 (1 5± 5 5 ) % ,PEF为 (11 3± 7 9) % ,两组比较差异有显著性 (P <0 0 1) ;(3 )运动后 10min及 1hA、B两组患者ECP、CD+ 2 5%、IL 5mRNA组间比较差异无显著性 (P均 >0 0 5 )。结论 嗜酸性粒细胞分泌的ECP。

Objective To explore the role of eosinophil cationic protein (ECP) and T lymphocytes in exercise induced asthma (EIA) Methods In 32 patients with asthma (13 patients with EIA) and 8 normal subjects, peripheral blood ECP,CD + 25 %,and IL 5 mRNA were measured before, 10 min and 60 min after exercise testing Results (1) There was a linear relationship between ECP,CD + 25 % and the forced expiratory volume in one second (FEV 1) before exercise ( r =-0 79, -0 61, P <0 01).(2) In patients with EIA, FEV 1 and PEF decreased remarkably after exercise test, but in patients without EIA, FEV 1 and PEF did not change The difference between the two groups was significant (3) There was no significent difference between the two groups in terms of ECP, CD + 25 %, and IL 5 mRNA. Conclusion T lymphocyte activation and ECP secreted by eosinophils do not seem to play a major role in EIA