期刊文献+

Chemical composition, mechanism of antibacterial action and antioxidant activity of leaf essential oil of Forsythia koreana deciduous shrub 被引量:15

Chemical composition, mechanism of antibacterial action and antioxidant activity of leaf essential oil of Forsythia koreana deciduous shrub
下载PDF
导出
摘要 Objective: To identify the chemical constituents of leaf essential oil of Forsythia koreana(F. koreana) and evaluate its ef ects on bacterial strains. Methods: The essential oil of leaf of F. koreana was extracted by using hydrodistillation process and the volatile components investigated with the help of gas chromatography coupled with mass spectrometry. The antibacterial study was carried out with the help of agar disc dif usion method, MIC, MBC and viable count. The mode of action was determined with help of potassium ion l ux, cellular material release and scanning electron microscopy. The antioxidant activity was determined with the help of 2, 3-diphenyl-2-picrylhydrazyl method, nitric oxide scavenging activity and superoxide anion radical scavenging assay. Results: Total ten compounds were identii ed as trans-phytol(42.73%), cis-3-hexenol(12.95%), 毬-linalool(10.68%), trans-2-hexenal(8.86%), trans-2-hexenol(8.86%), myrcenol(3.86%), 4-vinylphenyl acetate(3.86%),(4Z)-4,6-heptadien-1-ol(3.18%), lemonol(2.73%) and benzeneacetaldehyde(2.27%) by gas chromatography coupled with mass spectrometry. The antibacterial study was demonstrated that leaf essential oil of F. koreana act against foodborne and other pathogenic bacteria. The mode of action revealed that this essential oil acted on the cytoplasmic membrane, resulting in loss of integrity and increased permeability. In addition, leaf essential oil of F. koreana was shown to be rich in linalool, which contributes to improved antioxidant activity. Conclusions: These results show that leaf essential oil of F. koreana has great potential as a natural food preservative, antibacterial and antioxidant agent. Objective: To identify the chemical constituents of leaf essential oil of Forsythia koreana(F. koreana) and evaluate its ef ects on bacterial strains. Methods: The essential oil of leaf of F. koreana was extracted by using hydrodistillation process and the volatile components investigated with the help of gas chromatography coupled with mass spectrometry. The antibacterial study was carried out with the help of agar disc dif usion method, MIC, MBC and viable count. The mode of action was determined with help of potassium ion l ux, cellular material release and scanning electron microscopy. The antioxidant activity was determined with the help of 2, 3-diphenyl-2-picrylhydrazyl method, nitric oxide scavenging activity and superoxide anion radical scavenging assay. Results: Total ten compounds were identii ed as trans-phytol(42.73%), cis-3-hexenol(12.95%), 毬-linalool(10.68%), trans-2-hexenal(8.86%), trans-2-hexenol(8.86%), myrcenol(3.86%), 4-vinylphenyl acetate(3.86%),(4Z)-4,6-heptadien-1-ol(3.18%), lemonol(2.73%) and benzeneacetaldehyde(2.27%) by gas chromatography coupled with mass spectrometry. The antibacterial study was demonstrated that leaf essential oil of F. koreana act against foodborne and other pathogenic bacteria. The mode of action revealed that this essential oil acted on the cytoplasmic membrane, resulting in loss of integrity and increased permeability. In addition, leaf essential oil of F. koreana was shown to be rich in linalool, which contributes to improved antioxidant activity. Conclusions: These results show that leaf essential oil of F. koreana has great potential as a natural food preservative, antibacterial and antioxidant agent.
出处 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第9期682-688,共7页 亚太热带医药杂志(英文版)
关键词 ANTIBACTERIAL ACTIVITY Antioxidant ACTIVITY Cell material RELEASED Essential oil GC-MS Potassium ion flux Antibacterial activity Antioxidant activity Cell material released Essential oil GC-MS Potassium ion flux
  • 相关文献

参考文献20

  • 1Pascale Cossart,Alice Lebreton.A trip in the “New Microbiology” with the bacterial pathogen Listeria monocytogenes[J]. FEBS Letters . 2014
  • 2Glen C Ulett,Makrina Totsika,Kolja Schaale,Alison J Carey,Matthew J Sweet,Mark A Schembri.Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection[J]. Current Opinion in Microbiology . 2013
  • 3James Hamuel Doughari,Patrick Alois Ndakidemi,Izanne Susan Human,Spinney Benade.Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata[J]. Journal of Ethnopharmacology . 2012 (3)
  • 4Praveen Dahiya,Sharmishtha Purkayastha.Phytochemical screening and antimicrobial activity of some medicinal plants against multi-drug resistant bacteria from clinical isolates[J]. Indian Journal of Pharmaceutical Sciences . 2012 (5)
  • 5Hsiu-Mei Chiang,Hua-Hsien Chiu,Yen-Ming Lai,Ching-Yen Chen,Hung-Lung Chiang.Carbonyl species characteristics during the evaporation of essential oils[J]. Atmospheric Environment . 2010 (18)
  • 6Hwa-Jin Suh,Seong-Ryul Kim,Kyung-Seok Lee,Shin Park,Sun Chul Kang.Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda: Insecta) larvae[J]. Journal of Photochemistry & Photobiology, B: Biology . 2010 (2)
  • 7M Chandrasekaran,V Venkatesalu.Antibacterial and antifungal activity of Syzygium jambolanum seeds[J]. Journal of Ethnopharmacology . 2004 (1)
  • 8R.Pandey,A.Kalra,S.Tandon,N.Mehrotra,H. N.Singh,S.Kumar.Essential Oils as Potent Source of Nematicidal Compounds[J]Journal of Phytopathology (鈥?),2003(7鈥?).
  • 9Free Radical Scavenging Activities of Some Edible Fruit Seeds[J]. Pharmaceutical Biology . 2003 (3)
  • 10A Ultee,E.J Smid.Influence of carvacrol on growth and toxin production by Bacillus cereus[J]. International Journal of Food Microbiology . 2001 (3)

共引文献3

同被引文献160

引证文献15

二级引证文献113

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部