摘要
Objective:To study the mechanism and significance of pH change in the coronary artery microthrombosis of rats.Methods:After the sodium laurate-induced model of coronary artery microthrombosis of rats was constructed,the vascular endothelial cells were separated and then cultured in the mediums with different pH values for 24 h.Enzyme linked immunosorbent assay was used to detect the content of von Willebrand factor(vWF) in the medium;while the real-time PCR and western blot assay were used to detect the expression of fibrinogenlike protein 2(FGL2) at the mRNA and protein level.The comprehensive evaluation was performed to discuss the effect of pH change on the coronary artery microthrombosis of rats.Results:The expression level of vWF detected by enzyme linked immunosorbent assay was 336.67±24.95,311.33±14.98,359.67±39.63,354.67±49.01 and 332.00±33.42(pg/mL) respectively;while the expression of vWF in the model group was 570.00±57.94,524.67±57.94,437.00±95.38,415.33±44.38 and 444.67±74.31 respectively.Being cultured under the different pH values,the relative expression level of FGL2 mRNA in the model group was 7.93±0.93,6.70±0.70.5.03±0.32,5.13±0:40 and 5.57±0.83 respectively.Conclusions:The coronary artery microthrombosis of rats can cause the high expression and secretion of vWF.Meanwhile,FGL2 is also up-regulated in the thrombosis and such up-regulation is more significant in the condition with low pH,which indicates that the low-pH condition may be one of factors that contribute to the cardiovascular diseases.
Objective:To study the mechanism and significance of pH change in the coronary artery microthrombosis of rats.Methods:After the sodium laurate-induced model of coronary artery microthrombosis of rats was constructed,the vascular endothelial cells were separated and then cultured in the mediums with different pH values for 24 h.Enzyme linked immunosorbent assay was used to detect the content of von Willebrand factor(vWF) in the medium;while the real-time PCR and western blot assay were used to detect the expression of fibrinogenlike protein 2(FGL2) at the mRNA and protein level.The comprehensive evaluation was performed to discuss the effect of pH change on the coronary artery microthrombosis of rats.Results:The expression level of vWF detected by enzyme linked immunosorbent assay was 336.67±24.95,311.33±14.98,359.67±39.63,354.67±49.01 and 332.00±33.42(pg/mL) respectively;while the expression of vWF in the model group was 570.00±57.94,524.67±57.94,437.00±95.38,415.33±44.38 and 444.67±74.31 respectively.Being cultured under the different pH values,the relative expression level of FGL2 mRNA in the model group was 7.93±0.93,6.70±0.70.5.03±0.32,5.13±0:40 and 5.57±0.83 respectively.Conclusions:The coronary artery microthrombosis of rats can cause the high expression and secretion of vWF.Meanwhile,FGL2 is also up-regulated in the thrombosis and such up-regulation is more significant in the condition with low pH,which indicates that the low-pH condition may be one of factors that contribute to the cardiovascular diseases.
基金
supported by Medical Scientific Research Foundation of Guangdong Province(No.A2012242)
