甲基丙烯酸环氧丙酯的致突变性和致癌性研究

Mutagenesis and Carcinogenesis Induced by Glycidyl Methacrylate

甲基丙烯酸环氧两酯(GMA)是我们研究组近年发现的致突变物。GMA在Ames试验和小鼠骨髓微核试验得到阳性结果,GMA可与DNA共价结合,在体外淋巴细胞培养诱导UDS和抑制DNA复制。GMA对雄性小鼠生殖细胞也有遗传毒性。我们研究了GMA诱导的PBR322质粒TcR基因的限制酶酶谱,HPLC谱和DNA序列。结果提示,某些限制酶的酶切位点发生位移,GMA诱发缺失和插入,并存在序列专一性和热点。而且,我们发现GMA诱发叙利亚金黄地鼠胚胎细胞的恶性转化。

Glycidyl methacrylate ( GMA ) is a recently recognized mutagen by our research group. GMA had positive results in Ames test and mouse bone marrow micronucleus test.GMA bound with DNA convalently, induced UDS and inhibited DNA replication in lymphocyte in vitro. GMA was also genotoxic to germ cells of male mice in vivo. In order to explore the mutagenesis of GMA we analyzed the restriction enzyme maps, HPLC maps and DNA sequence of GMA-induced TcR(tetracycline-resistance) gene of plasmid PBR322. The results indicated that the recognition sites of some restriction enzymes changed,GMA induced the deletion and insertio , there were the sequence specificity and hot spot region. Moreover we found that GMA induced malignant transformation of syrian hamster embryo cells in vitro.