BCR连续提取法联合ICP-MS分析藏药白脉软膏及其矿物原料药中铅、镉、砷、汞、铜元素提取形态

Analysis of extracted forms of Pb, Cd, As, Hg and Cu in Tibetan medicine Baimai ointment and its mineral materials with BCR sequential extraction procedure combined with ICP-MS

目的:BCR连续提取白脉软膏及其矿物原料药(阳起石、碱花)中的铅、镉、砷、汞、铜元素。按照提取的难易程度划分为不同的提取形态,采用ICP-MS法对不同提取形态的各种元素进行测定,以此对样品中的不同提取形态各元素的含量和分布进行全方位分析。方法:白脉软膏及其矿物原料药(碱花、阳起石)样品经BCR连续提取法处理后,得到残渣态、水溶态、弱酸态、可还原态、可氧化态、总态等供试品溶液,以ICP-MS分别测定铅、镉、砷、汞、铜。结果:按检测样品统计,白脉软膏中铅、镉、砷、汞、铜元素多以残渣态、水溶态存在;阳起石中多以可还原态、残渣态存在;碱花中多以可还原态、可氧化态、残渣态存在。按元素提取形态统计,铅在样品中主要以残渣态、可还原态、可氧化态存在;镉主要以残渣态存在;砷主要以残渣态、弱酸态、可还原态存在;汞主要以可还原态、可氧化态、残渣态存在;铜主要以残渣态、弱酸态、可还原态存在。水溶态和弱酸态在阳起石的5种元素总和中占5.6%,碱花为19.94%,白脉软膏为15.12%。结论:在白脉软膏、阳起石和碱花中,5种元素(铅、镉、砷、汞、铜)在5种元素提取形态(水溶态、弱酸态、可还原态、可氧化态、残渣态)中均有检出,且含量具有比较明显的差异。单从检测数据角度分析,推测样品的安全性符合要求。

Objective:Pb,Cd,As,Hg and Cu in Baimai ointment and its mineral materials(actinolite and trona)were extracted by BCR sequential extraction procedure.According to the difficulty of the extraction,the different extracted forms were divided and determined by ICP-MS.In this way,the content and distribution of Pb,Cd,As,Hg and Cu in different extracted forms were analyzed comprehensively.Methods:After processed by BCR sequential extraction,the samples were divided into residual form,water-soluble form,weak acidulous form,reducible form,oxidable form and total form,then the contents of Pb,Cd,As,Hg and Cu were detected by ICP-MS.Results:According to the statistics of test samples,Pb,Cd,As,Hg and Cu in Baimai ointment were mainly in the forms of residual and watersoluble.Elements in actinolite were mainly in the forms of reducible and residual.And those in trona were mainly in the forms of reducible,oxidable and residual.According to the statistics of elemental extracted forms,Pb was mainly in forms of residual,reducible and oxidable,Cd was mainly in the form of residual,As was in forms of residual,weak acidulous and oxidable,Hg was mainly in forms of reducible,oxidable and residual,and Cu was mainly in forms of residual,weak acidulous and reducible.The water-soluble and weak acidulous forms accounted for 5.6%of the total amount of actinolite,19.94%of trona,and 15.12%of Baimai ointment.Conclusion:Five elements(Pb,Cd,As,Hg and Cu)were detected in the extraction forms(residual form,water-soluble form,weak acidulous form,reducible form,oxidable form)of Baimai ointment,actinolite and trona,and their contents were obviously different.From the perspective of test data,it is speculated that the safety of samples meets the requirements.