维生素C和亚硒酸钠对大鼠肝脏细胞色素P448的抗诱导作用

The Antiinducing Effect on Rat Liver CytochromeP-448 by Vitamin C and Sodium Selenite

化学致癌剂4,4’-亚甲基双(2-氯苯胺)[MOCA]可明显诱导雄性大鼠肝脏乙氧基异吩噁唑O-脱乙基酶[EROD]活性,该酶为细胞色素P448的标准酶。在用MOCA染毒前一小时腹腔注射维生素C(650mg/kg)或亚硒酸钠(1mg/kg),均可明显抑制EROD活性的诱导。连续经口灌胃给维生素C(150mg/kg)或亚硒酸钠(0.5mg/kg)三周,也可明显抑制MOCA对大鼠肝脏EROD活性的诱导。提示两种药物对化学致癌物在体内的代谢活化过程有干扰作用。

The chemical carcinogen 4, 4′-methylene bis ( 2-chloroaniline ) [MOCA] ( 150mg/kg ) can significantly induce liver microsome ethoxyresorufin O-deethylase ( EROD ) activity which specially represents the cytochrome P-448 in male rat. When rats were treated with vitamin C ( 650mg/kg ) or sodium selenite (1mg/kg ) via intraperitoneal injection before the MOCA t-reatment, the EPOD activity induced by MOCA was inhibited to normal level. While rats were administrated vitamin C ( 150mg/kg )or sodium selenite( 0.5mg/kg )via direct gastroga vage for 3 consecutive weeks, the inhibitory effect on the induced cytochrome P-448 activity was also observed. These results suggest that both vitamin C and sodium selenite have inte-rfence effects on the metabolizing active process of chemical carcinogens.