人参皂苷对酒精性肝损伤的保护作用研究

Protection of ginsenosides extract on alcoholic-induced hepatic injury of mice

目的研究人参皂苷提取物(GE)对酒精性肝损伤的保护作用与作用机制。方法将ICR雄性小鼠分为正常对照组、阳性对照组(联苯双酯)、模型组及人参皂苷提取物高、中、低剂量组,建立慢性酒精性肝损伤模型,用人参皂苷提取物进行干预,ig给药30 d后,称取肝质量,计算肝脏系数,测定小鼠血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活力、三酰甘油(TG)、肝脏内丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量,HE染色观察小鼠肝脏病理变化,综合评价人参皂苷提取物对小鼠酒精性肝损伤的保护作用。结果人参皂苷提取物各剂量组能明显降低酒精性肝损伤小鼠的肝脏系数(P<0.05)、血清TG的含量(P<0.01),可降低其血清中ALT、AST活力(P<0.05或P<0.01),但与正常组ALT、AST活力仍有差异(P<0.05);可在一定程度上降低肝组织中MDA含量(P<0.01),增高肝组织中GSH含量(P<0.01),减少肝组织病理损伤。结论人参皂苷提取物对酒精性肝损伤有一定保护作用,其机制可能与抑制肝内脂肪堆积,抗氧化作用有关。

Objective To investigate the hepatoprotective effects of ginsenosides extract(GE) on alcoholic-induced liver injury in mice. Methods ICR mice were randomly divided into six groups, including negative control group, positive control group(Bifendate), model group, and low-, mid-, high-dose GE groups. The model of alcoholic-induced liver injury was induced in ICR mice by 56° alcohol. The liver injury mice were intervened by GE for 30 d followed by liver weight and liver index. Meanwhile, the contents of serum alanine aminotransferase(ALT) and aspertate aminotransferase(AST) energy, triglyceride(TG), and malondialdehyde(MDA) and glutathione(GSH) in the liver were determined. The pathological changes in mice liver were also observed. Comprehensive evaluation of GE on the protective effects of alcoholic liver injury in mice was performed. Results GE could obviously reduce the liver weight coefficient of alcoholic-induced liver injury in mice(P < 0.05) and the content of TG in serum(P < 0.01). The levels of ALT and AST in serum could be reduced(P < 0.05 or 0.01), but it had difference from the normal group(P < 0.05). To a certain extent, GE can reduce the MDA content in liver tissue homogenate(P < 0.01), increase the content of GSH in liver tissue homogenate(P < 0.01) and reduce the liver tissue pathological damage. Conclusion GE has certain protective effect on alcoholic liver injury in mice. The inhibitory effect of fat accumulation in the liver is more apparent. Ginsenosides could protect liver through the mechanism of anti lipid peroxidation.