牛樟芝水提取物抗过敏作用及其机制研究

Anti-allergic effects of aqueous extract from Antrodia camphorata and its mechanism

目的研究牛樟芝水提物的抗过敏作用并初步探讨其作用机制。方法采用大鼠被动皮肤过敏模型、小鼠迟发型超敏模型、小鼠全身皮肤瘙痒模型、小鼠腹腔毛细血管通透性模型,观察牛樟芝水提物在体抗过敏作用。采用体外培养的RBL-2H3肥大细胞,观察牛樟芝水提物对细胞增殖及凋亡的影响,初步探讨其抗过敏反应的作用机制。结果体内试验中,牛樟芝水提物显著降低大鼠被动皮肤过敏反应,明显降低小鼠耳肿胀度、胸腺指数及脾指数,提高右旋糖酐所致皮肤瘙痒阈值,减少小鼠瘙痒次数,抑制组胺所致血管通透性上升;体外试验中,其剂量相关性的抑制RBL-2H3细胞增殖,促进细胞凋亡。结论牛樟芝水提取物有一定的抗过敏作用,其机制与促进RBL-2H3细胞凋亡有关。

Objective To explore the anti-allergic effects and possible mechanism of aqueous extract from Antrodia camphorata(EAC).Methods The anti-allergic effects of EAC were investigated in the passive cutaneous anaphylaxis(PCA)model of rat,in the delayed-type hypersensitivity(DTH)model of mice,in the dextran induced pruritus reaction model of mice,and in the peritoneal capillary permeability model of mice.RBL-2H3 mast cells were cultured in vitro to observe the effects of EAC on cell proliferation and apoptosis.Results EAC in vivo significantly reduced PCA reaction in rats,decreased the ear swelling degree,the index of thymus and spleen,and also significantly improved dextran-induced pruritic threshold and reduced the scratching number,and inhibited vascular permeability caused by histamine.EAC in vitro concentration-dependently inhibited RBL-2H3 mast cell proliferation and raised cell apoptosis level.Conclusion EAC has certain anti-allergic effects and its mechanism partially related to promote mast cell apoptosis.