葛根主要活性成分及作用机制的网络药理学研究

Investigating main components and action mechanism of Pueraria lobata based on pharmacology network

目的采用网络药理学的方法和思路研究葛根,探讨葛根成分-靶点、成分-疾病、基因-通路和基因-疾病的关联性,揭示其主要活性成分和药理作用机制。方法针对葛根中的活性成分,根据PubMed、中药系统药理学数据库和分析平台(TCMSP)数据库中获得葛根的化学成分和相关作用靶点,构建'成分-靶点'网络;运用UniProt和CTD数据库提取作用靶点的基因名称、信号通路、心血管疾病相关的基因和靶点数据进行富集分析。结果共获得刺芒柄花素、葛根素等10个主要活性成分;这些成分可作用于112个靶点,其主要靶点有PTGS2、PTGS1、HSP90AB1等,这些靶点与肿瘤、高血压、糖尿病等436种疾病相关,涵盖肿瘤、心脑血管疾病等33大类疾病。葛根主要活性成分作用于心血管疾病的有48个靶点,这些靶点参与肿瘤、高血压等多种生理及病理的生物学过程,如肿瘤坏死因子(TNF)等相关靶点,说明葛根是通过多成分、多靶点的协调作用发挥治疗疾病的作用。结论葛根具有抗肿瘤、降血压、降血脂等生物活性,具有较好的应用价值,为接下来的药效试验研究提供科学依据。

Objective To study the Pueraria lobata by system network pharmacology method,investigate the relations between main components-targets,targets-diseases and by analyzing the relationship of compounds-targets-gene-diseases,and reveal the multidimensional action pharmacology mechanism of Pueraria lobata.Methods The components of Pueraria lobata action targets and were obtained from TCMSP database and PubMed,and The components with OB≥20%and DL≥0.18 were the main active.The gene names of the targets,the pathways and corresponding diseases related with the targets were extracted from Uniprot database and CTD analysis platform online,respectively.At last,the components-targets,targets-diseases.Result The analysis of several networks pharmacological maps showed that The 10 active components were obtained,such as formononetin,puerarin,daidzein-4′,7-diglucoside,beta-sitosterol,3’-methoxydaidzein,daidzein,daidzin,ononin.There were 112 targets related with the 10 active components,and the main targets were PTGS2,PTGS1,HSP90 AB1 and so on.Those targets were related with 436 diseases including cancer,hypertension,diabetes mellitus,containing 33 kinds of diseases such as cancer,cardiovascular disease and so on.The main active components of Pueraria lobata have 48 targets acting on Cardiovascular disease.Those targets involved in various physiological and pathological processes such as cancer,Hypertension,Brain Ischemia,and inflammatory reaction in body,indicating that Pueraria lobata has multiple components and targets providing synergistic effects in the treatment of diseases.Conclusion Pueraria lobata has anti-cancer,lower blood pressure,and lower blood lipid avtivites,and could be used for treating cancer,cardiovascular disease,diabetes and so on,suggesting that Pueraria lobata has better application prospects.