羟丙基透明质酸的制备及性质研究

Study on Preparation and Properties of Hydroxypropyl Hyaluronic Acid

羟丙基透明质酸(HHA)是环氧丙烷对透明质酸(HA)的羟基修饰的产物。文章对HHA的制备方法及性质进行了研究,通过比较样品溶液在酶作用下粘度变化情况研究了其抗透明质酸酶降解的特性,并以相近相对分子质量的HA作对照考察了其细胞毒性。结果:建立了碱性条件下的HHA的制备方法,确定了HHA具有明显的抗透明质酸酶降解的特性,与HA相比两者的细胞毒性没有明显区别。

Hydroxypropyl hyaluronic acid( HHA) may be obtained by the modification of hydroxyl group of hyaluronic acid( HA)with epoxy propane. The aim of the paper is to study the preparation method and the property of degradation-resisting by hyaluronidase and the cytotoxicity of HHA. Comparison of the rangeability of the sample solution's viscosity with hyaluronidase the property of degradation-resisting by hyaluronidase was studied. Using HA with similar relative molecular mass as reference substance,the cytotoxicity of HHA was studied. The result indicates that the preparation method of HHA which is under a alkaline condition was established. Compared with HA,HHA has evident property of resistance to degrade by hyaluronidase and there is no obvious difference between them about cytotoxicity. The conclusion is that HHA is a safe biomaterial and has better property of degradation-resisting by hyaluronidase.