摘要
建立了翻白草及属间两种药用植物黄酮类化合物的HPLC指纹图谱。本文色谱条件为:色谱柱为Atlantis C18(250 mm×4.6 mm,5μm),流动相为甲醇-水(40∶60),体积流量为1.0 mL/min,柱温为25℃,检测波长:368 nm,进样量20μL。结果检测出10个分离度良好的共有指纹峰,计算了共有峰的相对保留时间和相对峰面积。结果表明该方法可作为科学评价及有效控制中药质量的可行的定性方法。
HPLC fingerprints,a sensitive and specific method is an enssential way to study the herb fingerprint. Atlantis C18( 250 mm ×4.6 mm,5 μm),flow rate: 1. 0 mL/ min,detecting wavelength: 368 nm and the column temperature: 25 ℃. The samples collected from different areas were determined. The samples quality was assessed by similarity evaluation system for chromatographic fingerprint and this method had a good repeatability and reproducibility. The method can be used to identify and evaluate the quality of Poterntilla discolor Bge conveniently.
出处
《药物生物技术》
CAS
2014年第3期250-252,共3页
Pharmaceutical Biotechnology
基金
湘西地区委陵菜属药用植物的ISSR分析及HPLC指纹图谱分析(湘财教指[2010]75号10C0442)