循环超声提取羊栖菜多糖的工艺优化

Optimization of Circulating Ultrasonic Extraction Polysaccharides from Sargassum Fusiforme

为研究循环超声提取分离羊栖菜多糖的中试工艺,实验以羊栖菜多糖提取率为指标,采用BoxBenhnken试验设计及响应面分析法优化循环超声提取的温度、固液比、功率、时间工艺参数,并对提取的粗多糖产品进行DPPH·清除的抗氧化评价。结果表明循环超声提取羊栖菜多糖的最佳工艺参数为:温度87℃、固液比1∶35(g/m L)、功率900 W、时间13 min。在此工艺参数下,羊栖菜多糖提取率可达10.54%,与预测值接近;所提取粗多糖产品得率为21.32%,DPPH·清除IC50值为6.64 mg。与传统热水浸提法相比,循环超声提取羊栖菜多糖提取率高,且提取时间短、粗多糖产品抗氧化性好,该工艺可为规模化生产羊栖菜多糖提供参考。

In order to study pilot procedure for circulating ultrasonic extraction of polysaccharides from Sargassum fusiforme,using the yield of polysaccharide as index,the following circulating ultrasonic process parameters: temperature,ratio of material to liquid,power and time were optimized by the method of Box-Benhnken design and response Surface Analysis. The anti-oxidation ability of crude polysaccharide to scavenge DPPH·was also investigated. The results indicated the optimal conditions were as follows: temperature 87 ℃,ratio of materials to liquid 1∶ 35,power 900 W and time for extraction 13 min. Under the optimal conditions,the extraction rate of Sargassum fusiforme Polysaccharides was up to 10. 54%,close to the predicted value and the yield of Sargassum fusiforme polysaccharides was up to 21. 32%. The IC50 of DPPH·scavenging for polysaccharide was 6. 64 mg. Compared with traditional method of water extraction,circulating ultrasonic extraction exhibits a higher extraction efficiency,short time consumption and produces crude polysaccharide with stronger capability to scavenge DPPH·. This process can provide a reference for large-scale production of Sargassum fusiforme polysaccharide.