β-淀粉样蛋白对海马神经元钙离子浓度的影响及其机制研究

Effect of Amyloid-β Protein on Intracellular Calcium Concentration in Cultured Hippocampal Neurons

目的 观察β-淀粉样蛋白 (Aβ1 -40 )对海马神经元内钙离子浓度的影响及尼莫地平的拮抗作用。方法 采用大鼠海马神经元的原代培养技术 ,分别用 MTT法和激光扫描共聚焦显微镜结合 Fluo-3 /AM标记观察神经元存活率和细胞内游离钙离子浓度变化。结果 Aβ1 -40在较高浓度 (1μmol/L和 1 0μmol/L )下 ,对神经元存活率和 [Ca2 + ]i 的影响与对照组相比 ,差异均有显著性 (P <0 .0 5 ,P <0 .0 1 ) ;5 μmol/L尼莫地平可部分降低Aβ1 -40引起的 [Ca2 + ]i 升高。结论 Aβ1 -40可引起培养海马神经元存活率下降及胞内钙离子浓度升高 ,尼莫地平有部分拮抗作用。

Objective To observe the effect of amyloid β protein(Αβ) on intracellular calcium concentration in cultured hippocampal neurons and the protective effect of nimodipine.Methods The cultured newborn rat hippocampal neurons were treated with aggregated Aβ1 40. MTT staining and LSCM was used to detected the survival rate and the intracellular free calcium concentration of neurons respectively.Results The survival rate was decreased and the fluorescence intensity was increased when neurons were treated with Aβ1 40 for 24 hours. There was obvious difference between exposed group and control group. Nimodipine could attenuate this effect partly.Conclusions Aβ1 40 reduce the survival rate and increase the intracellular free calcium concentration of neurons,nimodipe possesses certainly prolection.