小鼠持续吸入二手烟对尼古丁成瘾性的研究

Study on the addiction of nicotine in miceby continuous inhalation of second-hand smoke

目的探讨小鼠持续被动吸烟是否会产生尼古丁依赖性,以及对空间学习记忆能力和运动能力的影响。方法取20只雄性小鼠(18～22 g)随机分成两组,13只实验组与7只对照组,并用苦味酸区分,列表记录它们的初始体重。实验组小鼠给予烟雾暴露3次/d,40 min/次,空白对照组不做任何处理。持续10 d后,对所有小鼠进行条件性位置偏爱(CPP)、水迷宫定位航行和负重游泳实验。实验数据由统计学软件SPSS 21. 0进行非参数秩和检验分析。结果条件性位置偏爱实验(CPP)显示,实验组停留在有烟区的时间明显多于对照组,差异有统计学意义(Z=-3. 126,P<0. 01)。水迷宫定位航行实验显示,持续10 d被动吸烟会增强小鼠的空间学习记忆能力(χ~2=15. 169,P<0. 01)。被动吸烟小鼠体重及体重增长率明显低于空白对照组。结论小鼠被动吸烟可引发成瘾且伴有生长发育迟缓,空间记忆学习能力增强,运动能力没有明显改变。

Objective To explore whether continuous inhalation of second-hand smoke in mice produces nicotine dependence and has effect on spatial memory function as well as athletic ability. Methods Twenty male mice( 18 ~ 22 g) were randomly divided into two groups,passive smoking group( n = 13) and controlled group( no smoking)( n = 7). Two groups were distinguished by Picric acid. Their initial weight were recorded. The passive smoking group was exposed in smog3 times/d for 40 min/time. The controlled group did not receive any treatment. After ten days,two groups of mice were subjected to conditioned place preference( CPP),morris water maze task,loaded swimming task. Experimental data was analyzed by statistical software SPSS 21. 0 for nonparametric rank sum test. Results The conditional position preference( CPP) experiment showed that the duration of the experimental group staying in the smoking area was significantly more than that of the controlled group,and the difference between the experimental group and the controlled group was statistically significant( Z =-3. 126,P<0. 01). Water maze positioning navigation experiment showed that a continuous 10-day passive smoking could enhance the spatial learning and memory ability of mice( χ~2= 15. 169,P<0. 01). The body weight and weight gain rate of passive smoking mice were significantly lower than those of the blank control group. Conclusion Passive smoking can cause addiction and growth retardation. Spatial learning and memory ability is enhanced while sports ability of mice isn’ t changed obviously.