人参皂甙及其组分对心肌细胞缺氧、再给氧和心肌缺血再灌注损伤的保护作用

THE PROTECTIVE EFFECT OF GINSENOSIDES AND ITS COMPONENTS ON MYOCYTES ANOXIA/REOXYGENATION AND MYOCARDIAL REPERFUSION INJURY

人参总皂甙(83μg/ml)及其组分Rb+Ro(简称Rb+Ro)能保护大鼠乳鼠心肌细胞培养缺糖缺氧性损伤,减少再给氧损伤时乳酸脱氢酶(LDH)释放,降低离体大鼠心脏缺血再灌注损伤时肌酸磷酸激酶(CPK)释放。250μg/ml人参总皂甙和人参皂甙Rg以上组分(简称Rg)则促进心肌细胞LDH释放。说明较低剂量人参总皂甙和人参皂甙Rb+Ro组分对心肌缺氧和再给氧与再灌注有保护作用;人参总皂甙抗心肌缺血和再灌注损伤的有效组分为Rb+Ro。

We have shown previously that ginsenosides(G) possesses protective action against myocardial infarction. In order to estimate the possible effect of G and its component on anoxia/reoxygenation and ischemia/reperfusion damage, cultured myocytes from neonate rats and isolated rat heart were used in the present experiments.The results indicate that LDH release from myocytes during anoxia and reoxygenation ware significantly depressed by G (83 μg/ml) and its component Rb+Ro; but significantly enhanced by higher concentration (250 μg/ml) of G. The CPK release from isolated rat heart during reperfusion were significantly reduced by G and its component Rb+Ro (8.3 μg/ml) in the infusion solution.It was concluded that G and its component Rb+Ro can protect anoxia/reoxygenation and reperfusion injury of cultured myocytes and isolated rat heart. One of the active components is ginsenoside Rb fraction.