独活中的两个新倍半萜化合物及其抗炎活性研究

Anti-inflammatory activity of two new sesquiterpenoids from Radix Angelicae Pubescentis

采用Diaion HP-20大孔吸附树脂、Sephadex LH-20凝胶柱色谱、硅胶柱色谱、制备液相色谱等分离方法对独活(Radix Angelicae Pubescentis)醇提组分进行分离纯化,并采用高分辨质谱、1D和2D核磁共振谱等波谱技术进行化合物结构鉴定。结果从独活醇提组分中分离得到两个新倍半萜类化合物Angesesquid A (1)和Angesesquid B(2)。采用原代大鼠椎间盘软骨细胞体外炎症模型对化合物进行体外抗炎活性评价,结果发现,化合物1和2对该体外炎症模型细胞的增殖均无明显影响,它们都能明显抑制原代大鼠椎间盘软骨细胞体外炎症模型细胞中NO的释放,但对PGE2的释放无明显抑制作用。这说明化合物1和2一定程度上都能抑制炎症反应的发生。

Chemical constituents from the ethanol extract of Radix Angelicae Pubescentis was isolated and purified through Diaion HP-20 macroporous, silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Two new sesquiterpenoid derivatives were identified as angesesquid A(1) and angesesquid B(2), and their structures were determined. In vitro degeneration model of primary rat disc chondro‐cytes was used to evaluate the anti-inflammatory activity of these two compounds. The results showed that compounds 1 and 2 had no anti-proliferation effect. Both compounds inhibited the release of NO, but had no inhibitory activity for the release of PGE2. This finding implies that both of these two new sesquiterpenoids could moderately inhibit the inflammatory reaction to some extent.