江西10269例女性HPV感染情况及分型检测分析

Analysis of the HPV infection and genotyping distribution in 10 269 women in Jiangxi area

目的分析江西地区女性宫颈人乳头瘤病毒(human papillomavirus,HPV)感染率和各基因亚型的分布情况,为预防HPV感染和降低宫颈癌的发生提供科学依据。方法采用聚合酶链反应(PCR)体外扩增和反向点杂交技术,对2015年9月至2016年6月在我院就诊的10 269例女性宫颈脱落细胞标本进行共28种HPV基因亚型检测。结果 10 269例宫颈脱落细胞标本HPV总阳性率为29.98%,其中单一亚型感染率62.68%,多重亚型感染率37.32%;15种高危型感染率较高的为16、52和58型,10种低危型感染率较高的为40、44、81型;HPV感染的高峰年龄在50岁及以上人群,各年龄组间HPV感染阳性率差异有统计学意义(P<0.01)。结论江西女性HPV感染阳性率较高,加强HPV分型筛查检测,做到早防早治,对降低本地区宫颈癌的发病率有重要的作用。

Objective To analyze the prevalence of cervical infection with human papillomavirus(HPV) and distribution of gene subtypes in Jiangxi province, and provide a basis for preventing HPV infection and reducing the accident rate of cervical cancer. Methods 10 269 female patients who received cervical testing in our hospital from September 2015 to June 2016 were selected as the research objects. The cervical cytology samples were tested for 28 HPV-DNA subtypes by using PCR and reverse dot blot hybridization techniques. Results The HPV infection rate of 10 269 cervix cytology sample was 29.98%, in which the single subtype infection rate was 62.68%, and the multiple subtype infection rate was 37.32%. The highest infection rate of the 15 high-risk-subtype were type 16, type 52 and type 58. The highest infection rate of the 10low-risk-subtype were type 40, type 44 and type 81. The peak age of HPV infection was 50 and over 50 years old.The difference between different age groups in HPV infection rate was significant(P<0.01). Conclusion The positive infection rate of HPV among women in Jiangxi province was high. In order to decrease the morbidity of cervical cancer, we should enhance HPV subtype detection, early prevention and treatment.